Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro : Possible relevance to pathogenesis
Identifieur interne : 004E48 ( Main/Exploration ); précédent : 004E47; suivant : 004E49Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro : Possible relevance to pathogenesis
Auteurs : Chung Y. Cheung [Hong Kong] ; Leo L. M. Poon [Hong Kong] ; Iris H. Y. Ng [Hong Kong] ; Winsie Luk [Hong Kong] ; Sin-Fun Sia [Hong Kong] ; Mavis H. S. Wu [Hong Kong] ; Kwok-Hung Chan [Hong Kong] ; Kwok-Yung Yuen [Hong Kong] ; Siamon Gordon [Royaume-Uni] ; YI GUAN [Hong Kong] ; Joseph S. M. Peiris [Hong Kong]Source :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes, Animaux, Cellules cultivées, Chimiokine CCL2 (génétique), Chimiokine CCL2 (métabolisme), Chimiokine CXCL10, Chimiokines CXC (génétique), Chimiokines CXC (métabolisme), Cytokines (génétique), Cytokines (métabolisme), Humains, Macrophages (immunologie), Macrophages (virologie), Protéines virales (génétique), Protéines virales (métabolisme), RT-PCR, Réplication virale, Souris, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Séquençage par oligonucléotides en batterie, Virus du SRAS (pathogénicité).
- MESH :
- génétique : Chimiokine CCL2, Chimiokines CXC, Cytokines, Protéines virales.
- immunologie : Macrophages, Syndrome respiratoire aigu sévère.
- métabolisme : Chimiokine CCL2, Chimiokines CXC, Cytokines, Protéines virales.
- pathogénicité : Virus du SRAS.
- virologie : Macrophages, Syndrome respiratoire aigu sévère.
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Chemokine CCL2 (genetics), Chemokine CCL2 (metabolism), Chemokine CXCL10, Chemokines, CXC (genetics), Chemokines, CXC (metabolism), Coronavirus, Cytokine, Cytokines (genetics), Cytokines (metabolism), Gene Expression Profiling, Humans, Macrophage, Macrophages (immunology), Macrophages (virology), Mice, Microbiology, Oligonucleotide Array Sequence Analysis, Pathogenesis, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (pathogenicity), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Viral Proteins (genetics), Viral Proteins (metabolism), Virology, Virus Replication.
- MESH :
- chemical , genetics : Chemokine CCL2, Chemokines, CXC, Cytokines, Viral Proteins.
- chemical , metabolism : Chemokine CCL2, Chemokines, CXC, Cytokines, Viral Proteins.
- immunology : Macrophages, Severe Acute Respiratory Syndrome.
- pathogenicity : SARS Virus.
- virology : Macrophages, Severe Acute Respiratory Syndrome.
- Animals, Cells, Cultured, Chemokine CXCL10, Gene Expression Profiling, Humans, Mice, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Virus Replication.
Abstract
The pathogenesis of severe acute respiratory syndrome (SARS) remains unclear. Macrophages are key sentinel cells in the respiratory system, and it is therefore relevant to compare the responses of human macrophages to infections with the SARS coronavirus (SARS-CoV) and other respiratory viruses. Primary human monocyte-derived macrophages were infected with SARS-CoV in vitro. Virus replication was monitored by measuring the levels of positive- and negative-strand RNA, by immunofluorescence detection of the SARS-CoV nucleoprotein, and by titration of the infectious virus. The gene expression profiles of macrophages infected with SARS-CoV, human coronavirus 229E, and influenza A (H1N1) virus were compared by using microarrays and real-time quantitative reverse transcriptase PCR. Secreted cytokines were measured with an enzyme-linked immunosorbent assay. SARS-CoV initiated viral gene transcription and protein synthesis in macrophages, but replication was abortive and no infectious virus was produced. In contrast to the case with human coronavirus 229E and influenza A virus, there was little or no induction of beta interferon (IFN-β) in SARS-CoV-infected macrophages. Furthermore, SARS-CoV induced the expression of chemokines such as CXCL10/IFN-γ-inducible protein 10 and CCL2/monocyte chemotactic protein 1. The poor induction of IFN-β, a key component of innate immunity, and the ability of the virus to induce chemokines could explain aspects of the pathogenesis of SARS.
Affiliations:
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Cheung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Ng, Iris H Y" sort="Ng, Iris H Y" uniqKey="Ng I" first="Iris H. Y." last="Ng">Iris H. Y. Ng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Luk, Winsie" sort="Luk, Winsie" uniqKey="Luk W" first="Winsie" last="Luk">Winsie Luk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Sia, Sin Fun" sort="Sia, Sin Fun" uniqKey="Sia S" first="Sin-Fun" last="Sia">Sin-Fun Sia</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Mavis H S" sort="Wu, Mavis H S" uniqKey="Wu M" first="Mavis H. S." last="Wu">Mavis H. S. Wu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Gordon, Siamon" sort="Gordon, Siamon" uniqKey="Gordon S" first="Siamon" last="Gordon">Siamon Gordon</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Sir William Dunn School of Pathology, University of Oxford</s1><s2>Oxford</s2><s3>GBR</s3><sZ>9 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName><orgName type="university">Université d'Oxford</orgName></affiliation></author><author><name sortKey="Yi Guan" sort="Yi Guan" uniqKey="Yi Guan" last="Yi Guan">YI GUAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S. M." last="Peiris">Joseph S. M. Peiris</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chemokine CCL2 (genetics)</term><term>Chemokine CCL2 (metabolism)</term><term>Chemokine CXCL10</term><term>Chemokines, CXC (genetics)</term><term>Chemokines, CXC (metabolism)</term><term>Coronavirus</term><term>Cytokine</term><term>Cytokines (genetics)</term><term>Cytokines (metabolism)</term><term>Gene Expression Profiling</term><term>Humans</term><term>Macrophage</term><term>Macrophages (immunology)</term><term>Macrophages (virology)</term><term>Mice</term><term>Microbiology</term><term>Oligonucleotide Array Sequence Analysis</term><term>Pathogenesis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (pathogenicity)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term><term>Virology</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Animaux</term><term>Cellules cultivées</term><term>Chimiokine CCL2 (génétique)</term><term>Chimiokine CCL2 (métabolisme)</term><term>Chimiokine CXCL10</term><term>Chimiokines CXC (génétique)</term><term>Chimiokines CXC (métabolisme)</term><term>Cytokines (génétique)</term><term>Cytokines (métabolisme)</term><term>Humains</term><term>Macrophages (immunologie)</term><term>Macrophages (virologie)</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>RT-PCR</term><term>Réplication virale</term><term>Souris</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Séquençage par oligonucléotides en batterie</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chemokine CCL2</term><term>Chemokines, CXC</term><term>Cytokines</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chemokine CCL2</term><term>Chemokines, CXC</term><term>Cytokines</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chimiokine CCL2</term><term>Chimiokines CXC</term><term>Cytokines</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Macrophages</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Macrophages</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chimiokine CCL2</term><term>Chimiokines CXC</term><term>Cytokines</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Macrophages</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Macrophages</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chemokine CXCL10</term><term>Gene Expression Profiling</term><term>Humans</term><term>Mice</term><term>Oligonucleotide Array Sequence Analysis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Virus Replication</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Animaux</term><term>Cellules cultivées</term><term>Chimiokine CXCL10</term><term>Coronavirus</term><term>Cytokine</term><term>Humains</term><term>Macrophage</term><term>Pathogénie</term><term>Microbiologie</term><term>RT-PCR</term><term>Réplication virale</term><term>Souris</term><term>Séquençage par oligonucléotides en batterie</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pathogenesis of severe acute respiratory syndrome (SARS) remains unclear. Macrophages are key sentinel cells in the respiratory system, and it is therefore relevant to compare the responses of human macrophages to infections with the SARS coronavirus (SARS-CoV) and other respiratory viruses. Primary human monocyte-derived macrophages were infected with SARS-CoV in vitro. Virus replication was monitored by measuring the levels of positive- and negative-strand RNA, by immunofluorescence detection of the SARS-CoV nucleoprotein, and by titration of the infectious virus. The gene expression profiles of macrophages infected with SARS-CoV, human coronavirus 229E, and influenza A (H1N1) virus were compared by using microarrays and real-time quantitative reverse transcriptase PCR. Secreted cytokines were measured with an enzyme-linked immunosorbent assay. SARS-CoV initiated viral gene transcription and protein synthesis in macrophages, but replication was abortive and no infectious virus was produced. In contrast to the case with human coronavirus 229E and influenza A virus, there was little or no induction of beta interferon (IFN-β) in SARS-CoV-infected macrophages. Furthermore, SARS-CoV induced the expression of chemokines such as CXCL10/IFN-γ-inducible protein 10 and CCL2/monocyte chemotactic protein 1. The poor induction of IFN-β, a key component of innate immunity, and the ability of the virus to induce chemokines could explain aspects of the pathogenesis of SARS.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Oxfordshire</li></region><settlement><li>Oxford</li></settlement><orgName><li>Université d'Oxford</li></orgName></list><tree><country name="Hong Kong"><noRegion><name sortKey="Cheung, Chung Y" sort="Cheung, Chung Y" uniqKey="Cheung C" first="Chung Y." last="Cheung">Chung Y. Cheung</name></noRegion><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Luk, Winsie" sort="Luk, Winsie" uniqKey="Luk W" first="Winsie" last="Luk">Winsie Luk</name><name sortKey="Ng, Iris H Y" sort="Ng, Iris H Y" uniqKey="Ng I" first="Iris H. Y." last="Ng">Iris H. Y. Ng</name><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S. M." last="Peiris">Joseph S. M. Peiris</name><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><name sortKey="Sia, Sin Fun" sort="Sia, Sin Fun" uniqKey="Sia S" first="Sin-Fun" last="Sia">Sin-Fun Sia</name><name sortKey="Wu, Mavis H S" sort="Wu, Mavis H S" uniqKey="Wu M" first="Mavis H. S." last="Wu">Mavis H. S. Wu</name><name sortKey="Yi Guan" sort="Yi Guan" uniqKey="Yi Guan" last="Yi Guan">YI GUAN</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Gordon, Siamon" sort="Gordon, Siamon" uniqKey="Gordon S" first="Siamon" last="Gordon">Siamon Gordon</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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